Isoelectric Focussing Essay Example for Free

Isoelectric Focussing Essay Isoelectric Focussing The method of separating proteins according to their isoelectric points in a pH gradient is called isoelectric focusing. This technique was discovered by H.Svensson in Sweden. This method has a high resolution power because ordinary paper electrophoresis resolves plasma proteins into six bands where as isoelectric focusing resolves it into 40 bands. In conventional electrophoresis the pH between anode and cathode is constant and the positively charged ions migrate toward the cathode and the negative ions migrate toward anode. But in isoelectric focusing, a stable pH gradient is arranged. The pH gradually increases from anode to cathode. When a protein is introduced at a pH which is lower than its isoionic point, it will possess a net positive charge and will migrate in the direction of the cathode. Due to the presence of pH gradient, the net charge of the molecule changes due to ionization as it moves forward. When the protein encounters a pH where its net charge is zero, it will stop migrating. This is the isoelectric point of the protein. Every protein present in the mixture will migrate to its isoelectric point and stops its migration there itself. Thus, once a final stable focusing is reached, the resolution will be retained for a long time. Enzyme proteins resolved by IEF are then separated in a second dimension based on their molecular weight. To conduct this, IEF gel is extruded from the tube and placed lengthwise on a slab gel of polyacrylamide saturated with SDS. When an electric current is applied, the enzymeproteins migrate from the IEF gel into the SDS gel and then get separated according to their mass. This method helps in excellent separation of cellular enzyme-proteins. Uses: The two dimensional gel electrophoresis is used in developmental biochemistry to monitor the increase or decrease in the intensity of a spot representing as specific protein as a function of cell growth. It is a standard method of judging protein purity.

Myanmar: A Nation in Crisis Essay -- Essays Papers

Myanmar: A Nation in Crisis The nation of Myanmar, also known as Burma, is currently under the rule of a ruthless totalitarian regime, guilty of numerous human rights violations and target of intense international criticism. Located in Southeast Asia, on the western border of Thailand and Laos, it has been under military rule since World War II. Burma is mired in socioeconomic crisis stemming from the rule of the military junta, and the citizens are suffering. The environment of Burma is being destroyed, the people are treated inhumanely, and the country is notorious for its contribution to global narcotics. The paragraphs below detail the current situations facing the country, why they came about, and the parties that are to blame for sending the country on a dangerous downward spiral. First, however, it is important to understand Burma’s political history. A History of Burma The ancestors of the people of Myanmar migrated down along the Irrawaddy River from Tibet and China, and they brought with them cultural traditions stemming from India. The Mon, at around 3000 B.C., were the first people to come. They established centers of settlement in central Myanmar, in the Irrawaddy delta, and farther down the eastern coast of the Bay of Bengal. They developed farming and irrigation systems, and they kept ties with India as well as other Mon settlements. The Burmese entered the Irrawaddy River valley in the mid-9th century, eventually absorbing the nearby Pyu and Mon communities. The Shan, Kachin, and Karen arrived later and are currently the major ethnic minority groups in the country. In 1044, King Anawrahta united the nation in the city of Pagan where he built Buddhist temples and pagodas. In the following years Burma expe... ...ive. 15 Nov. 2004 . Thein, Myat. Economic Development of Myanmar. Singapore: Institute of Southeast Asian Studies, 2004. U.S. Department of State. International Narcotics Control Strategy Report, 1998. Washington, DC: U.S. Department of State, 1999. ---. International Narcotics Control Strategy Report, 2000. Washington, DC: U.S. Department of State, 2001. Waldman, Peter. â€Å"Pipeline Project in Burma Puts Cheney in the Spotlight.† The Wall Street Journal. 27 Oct. 2000 < http://www.burmaforumla.org/news/cheney.htm>. Wallensteen, Peter. Routes to Democracy in Burma/Myanmar. Uppsala, Sweden: Dept. of Peace and Conflict Research, Uppsala University, 2004.

Bill Gates and DOS Operating System

Bill Gates has proven himself to be the wealthiest man alive. He initiated the monster computer software business called Microsoft and has produced the most widely used computer software programs to date. Bill Gates is a transgressive person meaning he has both helped society progressively and regressively. Bill Gates has progressively helped our society by introducing the basic operating system DOS in 1983. This basic operating system consisted of a single prompt, which allowed the user to complete tasks by typing in the right command line. When the press asked Gates what it took to create the Dos operating system, he replied, â€Å"Well, to create a new standard, it takes something that†s not just a little bit different. It takes something that†s really new and really captures people†s imagination.† (Microsoft Conference on Dos, 1984) When people saw the operating system, they were awestruck. Over ten million copies of Dos where sold during the first two years of sales. Society benefited from this operating system. By using it to run programs easily and efficiently, the Dos network allowed a whole new frontier of micro-management for businesses. Transactions could be saved to hard drives and easily located for later use. The average home computer user would have to take computer courses to learn the basic operations of the interfaces, which were available at the time. Dos offers a short instruction manual and also a set of easily understood operations. After Microsoft had gained control of the market with the Dos operating system, Bill Gates and his company Microsoft started on a new program that again would change the market. This alteration would not be in the home computer region, but the programming industry. The program was called the OS/2. â€Å"I believe Os/2 is destined to be the most important operating system, and possibly program, of all time. As the successor to Dos, which has over 10,000,000 system in use, it creates opportunities for everyone involved in Pc†s.† (Bill Gates, Os/2 Programmer†s guide). The program was a simple interface which made creating programs efficient and easy. Low-level programmers could write unthinkable programs in a fraction of the time it would take a team of programmers to complete programs. With this technology, programs that would help our society where brought to our reach faster. After setting up these two major projects, Bill Gates and Microsoft released windows 1.0. This version of Microsoft†s operating system was the first of many steps in the evolution of Microsoft being the monopoly it is today. The windows operating system allowed non-computer lingual people to manipulate a computer with ease. The progressive program allowed families to use their home computers to educate and entertain themselves. The major flaw with the windows operating system was that Bill gates and Microsoft produced windows with the basic drop-down menu. Macintosh had already copyrighted this, later; they brought Microsoft to court over the matter and won their settlement. With Microsoft windows being run on most PC†s, Bill Gates and Microsoft began work for upgrades. Through the evolution of technology, Microsoft has made four new version of the windows operating system, each one bettering the last. The interface has gotten more and more user-friendly. With the most recent version, windows 2000, you can actually learn all of the operations through the program†s tutorial. This self-education application allows easy understanding and use for the users. The Internet has attracted many people to buy a computer and with the windows operating system, users will have no problem adapting to the computer environment. One of the more recent programs that Microsoft has initiated is the Internet explorer. This program has progressively helped many businesses start web pages and has also allowed families get connected to the Internet. While the Internet explorer does help our society progressively, at the same time, it regressively helped the workers of Netscape Navigator. Microsoft included the Internet explorer free with every windows operating system package, starting from Windows 95 and up. Microsoft single handedly took control of the Internet browsing business. This made Netscape, the makers of Netscape navigator; make cutbacks in regards to their employees. Netscape brought Microsoft to court and won. Although the courts decision was guilty, it has not affected direct sales of their product. Bill Gates has also affected our society in regressive or negative ways. Over the course of Bill Gate†s working career with Microsoft, he has accumulated a wealth of $119.704 billion dollars. The U.S, population is only 274,231,556 as of Thursday, February 10, 2000. This means that each person has contributed $436.507 dollars toward his wealth, whether or not they are buying Microsoft products or not. While many people do not even own a computer, they have contributed to Microsoft through taxes and other means of financial Microsoft in itself is a regressive business when looked at through the eyes of a computer software business. Bill Gates almost ruined Macintosh Computer, but decided that it would be better to own them, rather then end the business. He now has turned them into an even larger corporation then before. † The next generation of interesting software will be made on a Macintosh, not on an IBM PC.† (Bill Gates, 1996) Bill Gates meant that he believes that the Macintosh will be the computer for tomorrow, not an IBM PC. By owning a large chunk of the Computer software industry, Microsoft has become a national monopoly. Most small time businesses cannot offer competition to a corporation such as Microsoft. Finally, Bill Gates has had a transgressive hold on society ever since the Microsoft Corporation began. He has helped our society by making the operating systems made by Microsoft and the other programs offered, available to us. Through the operating systems: stocks have risen, people are becoming open to the world through the Internet, and important medical research can be accomplished as well as hundreds of other useful task.

John Maynard Keynes s Law Starting With Aggregate Demand...

â€Å"There is no inherent reason to believe that investment outlays plus consumption outlays would always tend to equal the cost of any given output; there is no assurance that Demand would tend to equal any given Supply† In order to discuss the statement in the title, I will first talk about J. M. Keynes and give some general information regarding his life and career. Following I will discuss about Keynes criticism of Say’s Law starting with Aggregate Demand and how consumption together with investment are in relation to income. Afterwards I will highlight the role of investment and what the policy implications are. For the final part of this essay I will conclude with some evidence to support the claims made. John Maynard Keynes was born in 5th of June 1883 and died at the age of 62 on the 21st of April 1946. His work in economics and his ideas fundamentally changed the practice and theory of modern macroeconomics as well as the economic policies of governments. Keynes is very well known for his exceptional work on the implications and causes of the business cycles and is also regarded as the founder of modern macroeconomics. The school of thought also known as ‘Keynesian economicsâ₠¬â„¢ as well as the various offshoots have his ideas as foundation. Keynes initiated a revolution in economic thinking by challenging the beliefs that neoclassical economists held. He argued that their ideas that free markets would naturally provide full employment in the short to medium term isShow MoreRelatedEconomics : Classical Economics And Keynesian Economics1665 Words   |  7 Pagesnot like riding a bike, once you know how to do it you will always have it engraved in your head. I will attempt to highlight the key factors of the two theories of economics: classical economics and Keynesian economics. Since Classical Economics is considered to be the first school of economics. I will start to explain this concept first. 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